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Immunohistochemical staining of human Skin shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human Skin shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human Skin shows moderate nuclear positivity in squamous epithelial cells.

Anti-TREX2 Antibody

HPA054060
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetTREX2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TREX2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11219
  • Target name
    TREX2
  • Target description
    three prime repair exonuclease 2
  • Target synonyms
    3'-5' exonuclease TREX2 long form; three prime repair exonuclease 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BQ50
  • Protein Name
    Three prime repair exonuclease 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TREX2 using anti-TREX2 antibody (PB10105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: MCF-7 whole cell lysates lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREX2 antigen affinity purified polyclonal antibody (Catalog # PB10105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREX2 at approximately 37 kDa. The expected band size for TREX2 is at 31 kDa.
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