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Immunohistochemical staining of human spleen shows cytoplasmic positivity in white pulp and sinusoids.
Immunohistochemical staining of human spleen shows cytoplasmic positivity in white pulp and sinusoids.
Immunohistochemical staining of human spleen shows cytoplasmic positivity in white pulp and sinusoids.

Anti-TRIB2 Antibody

HPA001305
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetTRIB2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRIB2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID28951
  • Target name
    TRIB2
  • Target description
    tribbles pseudokinase 2
  • Target synonyms
    C5FW; GS3955; TRB2; tribbles homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92519
  • Protein Name
    Tribbles homolog 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRIB2 using anti-TRIB2 antibody (PB9900). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIB2 antigen affinity purified polyclonal antibody (Catalog # PB9900) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIB2 at approximately 39 kDa. The expected band size for TRIB2 is at 39 kDa.
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