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Immunohistochemical staining of human placenta shows distinct positivity in endothelial cells.
Immunohistochemical staining of human placenta shows distinct positivity in endothelial cells.
Immunohistochemical staining of human placenta shows distinct positivity in endothelial cells.

Anti-TRIM6 Antibody

HPA060514
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRIM6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRIM6 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID117854
  • Target name
    TRIM6
  • Target description
    tripartite motif containing 6
  • Target synonyms
    RNF89, tripartite motif-containing protein 6, RING finger protein 89, RING-type E3 ubiquitin transferase TRIM6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9C030
  • Protein Name
    Tripartite motif-containing protein 6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TRIM6 using anti-TRIM6 antibody (A02016-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K56 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM6 antigen affinity purified polyclonal antibody (Catalog # A02016-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM6 at approximately 56KD. The expected band size for TRIM6 is at 56KD.
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