Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM208 at 1 ug/mL, showed a band of Histone H4 trimethylated at Lysine20 in HeLa cells.
Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM208 at 1 ug/mL, showed a band of Histone H4 trimethylated at Lysine20 in HeLa cells.
anti-Trimethyl-Histone H4 (Lys20), Rabbit Monoclonal (RM208)
REV-31-1096-00
ApplicationsWestern Blot, ELISA, Other Application
Product group Antibodies
ReactivityVertebrate
TargetH4-16
Overview
- SupplierRevMAb Biosciences
- Product Nameanti-Trimethyl-Histone H4 (Lys20), Rabbit Monoclonal (RM208)
- Delivery Days Customer5
- ApplicationsWestern Blot, ELISA, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM208
- Concentration1 mg/ml
- Gene ID121504
- Target nameH4-16
- Target descriptionH4 histone 16
- Target synonymsH4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; HIST4H4; histone 4, H4; histone cluster 4 H4; histone H4
- HostRabbit
- IsotypeIgG
- Scientific DescriptionHistone H4 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H4 is involved with the structure of the nucleosome of the beads on a string organization. Histone proteins are highly post-translationally modified. Covalently bonded modifications include acetylation and methylation of the N-terminal tails. These modifications may alter expression of genes located on DNA associated with its parent histone octamer. Histone H4 is an important protein in the structure and function of chromatin, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. - Recombinant Antibody. This antibodyreacts to Histone H4 trimethylated at Lysine 20 (K20me3). It may slightly cross react to dimethylated Lysine 20 (K20me2) at a higher concentration. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4. Applications: WB, ELISA, Multiplex. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. Histone H4 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H4 is involved with the structure of the nucleosome of the beads on a string organization. Histone proteins are highly post-translationally modified. Covalently bonded modifications include acetylation and methylation of the N-terminal tails. These modifications may alter expression of genes located on DNA associated with its parent histone octamer. Histone H4 is an important protein in the structure and function of chromatin, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.
- ReactivityVertebrate
- Storage Instruction-20°C
- UNSPSC12352203