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Anti-TrkA [MNAC13]

Ab00439-1.1
Absolute Antibody
ApplicationsELISA, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application
Product group Antibodies
TargetNTRK1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-TrkA [MNAC13]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody binds to human TrkA, a receptor tyrosine kinase involved in the development and the maturation of the CNS and the PNS through regulation of proliferation, differentiation, and survival of the sympathetic and nervous neurons. It is a receptor for NGF and phosphorylates several downstream proteins regulating a variety of signalling cascades. This antibody neutralizes the NGF-TrkA interation.
  • Applications
    ELISA, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application
  • Applications Supplier
    ELISA; SPR; Blocking; IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MNAC13
  • Gene ID4914
  • Target name
    NTRK1
  • Target description
    neurotrophic receptor tyrosine kinase 1
  • Target synonyms
    gp140trk; high affinity nerve growth factor receptor; MTC; neurotrophic tyrosine kinase, receptor, type 1; Oncogene TRK; p140-TrkA; TRK; TRK1; TRK1-transforming tyrosine kinase protein; TRKA; Trk-A; tropomyosin-related kinase A; tyrosine kinase receptor A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP04629
  • Protein Name
    High affinity nerve growth factor receptor
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Human TrkA ECD.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TrkA using anti-TrkA antibody (A00706). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TrkA antigen affinity purified polyclonal antibody (Catalog # A00706) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TrkA at approximately 150KD. The expected band size for TrkA is at 87KD.
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