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Immunohistochemical staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.

Anti-TRMT10C Antibody

HPA036671
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRMT10C
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRMT10C
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54931
  • Target name
    TRMT10C
  • Target description
    tRNA methyltransferase 10C, mitochondrial RNase P subunit
  • Target synonyms
    COXPD30, HNYA, MRPP1, RG9MTD1, tRNA methyltransferase 10 homolog C, HBV pre-S2 trans-regulated protein 2, RNA (guanine-9-) methyltransferase domain containing 1, mRNA methyladenosine-N(1)-methyltransferase, mitochondrial RNase P subunit 1, mitochondrial ribonuclease P protein 1, renal carcinoma antigen NY-REN-49, tRNA (adenine(9)-N(1))-methyltransferase, tRNA (guanine(9)-N(1))-methyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7L0Y3
  • Protein Name
    tRNA methyltransferase 10 homolog C
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TRMT10C using anti-TRMT10C antibody (A09612-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT10C antigen affinity purified polyclonal antibody (Catalog # A09612-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT10C at approximately 40-45 kDa. The expected band size for TRMT10C is at 47 kDa.
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