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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Anti-TRMT44 Antibody

HPA045461
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRMT44
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRMT44 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID152992
  • Target name
    TRMT44
  • Target description
    tRNA methyltransferase 44 homolog
  • Target synonyms
    C4orf23, METTL19, TRM44, probable tRNA (uracil-O(2)-)-methyltransferase, putative tRNA (uracil-O(2)-)-methyltransferase, methyltransferase like 19, methyltransferase-like protein 19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IYL2
  • Protein Name
    Probable tRNA (uracil-O(2)-)-methyltransferase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRMT44 using anti-TRMT44 antibody (A14732-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT44 antigen affinity purified polyclonal antibody (Catalog # A14732-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT44 at approximately 85 kDa. The expected band size for TRMT44 is at 85 kDa.
Product group Antibodies
Boster Bio
Anti-TRMT44 Antibody Picoband(r)A14732-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetTRMT44
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Product group Antibodies
LifeSpan BioSciences
METTL19 Antibody (aa427-476)LS-C111461
ApplicationsWestern Blot
ReactivityHuman
TargetTRMT44
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Product group Antibodies
GeneTex
C4orf23 antibodyGTX119915
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
ReactivityHuman
TargetTRMT44
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Atlas Antibodies
PrEST Antigen TRMT44APREST79798
Protein IDQ8IYL2
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