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Immunohistochemical staining of human heart muscle shows strong cytoplasmic and nuclear positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic and nuclear positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic and nuclear positivity in myocytes.

Anti-TRPC7 Antibody

HPA031126
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRPC7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRPC7
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57113
  • Target name
    TRPC7
  • Target description
    transient receptor potential cation channel subfamily C member 7
  • Target synonyms
    likley ortholog of mouse transient receptor potential cation channel, subfamily C, member 7; putative capacitative calcium channel; short transient receptor potential channel 7; transient receptor protein 7; TRP7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HCX4
  • Protein Name
    Short transient receptor potential channel 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRP 7/TRPC7 using anti-TRP 7/TRPC7 antibody (A04658-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human SK-N-SH whole cell lysates, Lane 5: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRP 7/TRPC7 antigen affinity purified polyclonal antibody (Catalog # A04658-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRP 7/TRPC7 at approximately 100 kDa. The expected band size for TRP 7/TRPC7 is at 100 kDa.
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