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Immunofluorescent staining of human cell line PC-3 shows localization to nuclear membrane.
Immunofluorescent staining of human cell line PC-3 shows localization to nuclear membrane.
Immunofluorescent staining of human cell line PC-3 shows localization to nuclear membrane.

Anti-TRPM2 Antibody

HPA030976
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetTRPM2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRPM2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7226
  • Target name
    TRPM2
  • Target description
    transient receptor potential cation channel subfamily M member 2
  • Target synonyms
    EREG1; estrogen-responsive element-associated gene 1 protein; KNP3; long transient receptor potential channel 2; LTRPC2; LTrpC-2; NUDT9H; NUDT9L1; transient receptor potential cation channel subfamily M member 2; transient receptor potential channel 7; transient receptor potential melastatin 2; TRPC7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94759
  • Protein Name
    Transient receptor potential cation channel subfamily M member 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western blot All lanes: TRPM2 antibody at 3microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse brain tissue Lane 3: A549 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: Mouse kidney tissue Lane 6: Human placenta tissue Lane 7: Mouse spleen tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 172, 166, 96 kDa Observed band size: 70 kDa
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Mouse probed with Rabbit Anti-TRPM2 Polyclonal Antibody, Unconjugated (bs-2888R) (green) at 1:100 for 40 minutes followed by a FITC conjugated secondary compared to control cells (blue).
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Immunohistochemistry analysis in human lymph node and kidney tissues using Anti-TRPM2 antibody. Corresponding TRPM2 RNA-seq data are presented for the same tissues.
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Figure 1. Western blot analysis of TRPM2 using anti-TRPM2 antibody (A01013-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM2 antigen affinity purified polyclonal antibody (Catalog # A01013-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM2 at approximately 171 kDa. The expected band size for TRPM2 is at 171 kDa.
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