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Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.
Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.
Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.

Anti-TRPM7 Antibody

HPA035522
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetTRPM7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TRPM7 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54822
  • Target name
    TRPM7
  • Target description
    transient receptor potential cation channel subfamily M member 7
  • Target synonyms
    ALSPDC, CHAK, CHAK1, LTRPC7, LTrpC-7, TRP-PLIK, transient receptor potential cation channel subfamily M member 7, LTRPC ion channel family member 7, channel-kinase 1, long transient receptor potential channel 7, transient receptor potential-phospholipase C-interacting kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96QT4
  • Protein Name
    Transient receptor potential cation channel subfamily M member 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TRPM7 using anti-TRPM7 antibody (A00789-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human COLO-320 whole cell lysate, Lane 3: human 22RV1 whole cell lysate, Lane 4: human SGC-7901 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM7 antigen affinity purified polyclonal antibody (Catalog # A00789-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM7 at approximately 250KD. The expected band size for TRPM7 is at 212KD.
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