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Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.

Anti-TSC22D1 Antibody

HPA077414
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetTSC22D1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TSC22D1
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8848
  • Target name
    TSC22D1
  • Target description
    TSC22 domain family member 1
  • Target synonyms
    HUCEP-2, Ptg-2, TGFB1I4, TSC22, TSC22 domain family protein 1, TGFB-stimulated clone 22 homolog, TGFbeta-stimulated clone 22, cerebral protein 2, regulatory protein TSC-22, transcriptional regulator TSC-22, transforming growth factor beta-1-induced transcript 4 protein, transforming growth factor beta-stimulated protein TSC-22
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15714
  • Protein Name
    TSC22 domain family protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TSC22D1 using anti-TSC22D1 antibody (A06397-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSC22D1 antigen affinity purified polyclonal antibody (Catalog # A06397-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSC22D1 at approximately 140-150 kDa. The expected band size for TSC22D1 is at 140-150 kDa.
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