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Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.

Anti-TSEN54 Antibody

HPA024543
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetTSEN54
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TSEN54 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID283989
  • Target name
    TSEN54
  • Target description
    tRNA splicing endonuclease subunit 54
  • Target synonyms
    PCH2A, PCH4, PCH5, SEN54L, sen54, tRNA-splicing endonuclease subunit Sen54, SEN54 homolog, TSEN54 tRNA splicing endonuclease subunit, hsSen54, tRNA-intron endonuclease Sen54
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z6J9
  • Protein Name
    tRNA-splicing endonuclease subunit Sen54
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TSEN54 using anti-TSEN54 antibody (A07022-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSEN54 antigen affinity purified polyclonal antibody (Catalog # A07022-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSEN54 at approximately 59 kDa. The expected band size for TSEN54 is at 59,20 kDa.
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