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Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Anti-TTC39A Antibody

HPA028098
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTTC39A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TTC39A
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22996
  • Target name
    TTC39A
  • Target description
    tetratricopeptide repeat domain 39A
  • Target synonyms
    C1orf34, DEME-6, tetratricopeptide repeat protein 39A, TPR repeat protein 39A, differentially expressed in MCF-7 with estradiol protein 6, differentially expressed in MCF7 with estradiol protein 6, testicular tissue protein Li 213
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5SRH9
  • Protein Name
    Tetratricopeptide repeat protein 39A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of TTC39A using anti-TTC39A antibody (A16108). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC39A antigen affinity purified polyclonal antibody (Catalog # A16108) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC39A at approximately 72 kDa. The expected band size for TTC39A is at 70 kDa.
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