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Immunofluorescent staining of human cell line U2OS shows localization to nuclear bodies.
Immunofluorescent staining of human cell line U2OS shows localization to nuclear bodies.
Immunofluorescent staining of human cell line U2OS shows localization to nuclear bodies.

Anti-TTC9 Antibody

HPA079473
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetTTC9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TTC9 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23508
  • Target name
    TTC9
  • Target description
    tetratricopeptide repeat domain 9
  • Target synonyms
    TTC9A, tetratricopeptide repeat protein 9A, TPR repeat protein 9A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92623
  • Protein Name
    Tetratricopeptide repeat protein 9A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TTC9 using anti-TTC9 antibody (A14174). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC9 antigen affinity purified polyclonal antibody (Catalog # A14174) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC9 at approximately 27 kDa. The expected band size for TTC9 is at 24 kDa.
Product group Antibodies
Boster Bio
Anti-TTC9 Antibody Picoband(r)A14174-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetTTC9
  • SizePrice