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Immunohistochemical staining of human ovary shows cytoplasmic positivity in follicle cells.
Immunohistochemical staining of human ovary shows cytoplasmic positivity in follicle cells.
Immunohistochemical staining of human ovary shows cytoplasmic positivity in follicle cells.

Anti-TTLL3 Antibody

HPA051413
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetTTLL3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TTLL3
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26140
  • Target name
    TTLL3
  • Target description
    tubulin tyrosine ligase like 3
  • Target synonyms
    HOTTL; tubulin monoglycylase TTLL3; tubulin tyrosine ligase-like family, member 3; tubulin--tyrosine ligase-like protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TTLL3 using anti-TTLL3 antibody (A11845-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL3 antigen affinity purified polyclonal antibody (Catalog # A11845-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTLL3 at approximately 87 kDa. The expected band size for TTLL3 is at 87 kDa.
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IHC image of CSB-PA23329A0Rb diluted at 1:300 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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