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Anti-TUBA1C (C-term) Antibody

102-21012
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTUBA1C
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-TUBA1C (C-term) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB31971
  • Gene ID84790
  • Target name
    TUBA1C
  • Target description
    tubulin alpha 1c
  • Target synonyms
    alpha-tubulin 6; bcm948; TUBA6; tubulin alpha-1C chain; tubulin alpha-6 chain; tubulin, alpha 6
  • Host
    Rabbit
  • Protein IDP08537
  • Protein Name
    Tubulin alpha chain
  • Scientific Description
    Rabbit Anti-TUBA1C (C-term) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tubulin Alpha using anti-Tubulin Alpha antibody (A03989-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tubulin Alpha antigen affinity purified polyclonal antibody (Catalog # A03989-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tubulin Alpha at approximately 55 kDa. The expected band size for Tubulin Alpha is at 50 kDa.
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