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Immunohistochemical staining of human testis shows weak to moderate cytoplasmic positivity.
Immunohistochemical staining of human testis shows weak to moderate cytoplasmic positivity.
Immunohistochemical staining of human testis shows weak to moderate cytoplasmic positivity.

Anti-UBAP2 Antibody

HPA021684
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetUBAP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-UBAP2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55833
  • Target name
    UBAP2
  • Target description
    ubiquitin associated protein 2
  • Target synonyms
    UBAP-2, ubiquitin-associated protein 2, AD-012 protein, RNA polymerase II degradation factor UBAP2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5T6F2
  • Protein Name
    Ubiquitin-associated protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of UBAP2 using anti-UBAP2 antibody (A12600-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBAP2 antigen affinity purified polyclonal antibody (Catalog # A12600-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBAP2 at approximately 117 kDa. The expected band size for UBAP2 is at 117 kDa.
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