Anti-Ubiquitin Rabbit mAb (N-terminal, BSA and Azide Free)

PTM BIO

Anti-Ubiquitin Rabbit mAb (N-terminal, BSA and Azide Free)

5

Anti-Ubiquitin Rabbit mAb (BSA and Azide Free) detects endogenous ubiquitin, polyubiquitin, and ubiquitinated proteins.

Western Blot

WB

Antibody

Research Use Only

Monoclonal

PDPTM-O0402

Unconjugated

Rabbit

IgG

Ubiquitin-40S ribosomal protein S27a

Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating a wide range of cellular processes. Through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitin covalently attaches to target proteins and marks them for proteasomal degradation, alters protein-protein interactions, modulates membrane protein trafficking, and controls the activity of many signal transduction pathways. Mono-ubiquitination of Histone H2B at Lys120 (H2BK120ub) serves signaling purposes and is reversible by ubiquitin-specific proteases (USPs). The enzymes responsible for H2BK120ub are Rad6 E2 proteins (HR6A and HR6B in human) and Bre1 E3 ligase (RNF20 and RNF40 in human). This modification triggers a “trans-histone” modification cascade, resulting in trimethylation of Lys4 and Lys79 in histone H3, which promotes gene activity.

Stable for 12 months from date of receipt/reconstitution.

All Species

All

Protein A purified

Before reconstitution, centrifuge the vial at 12,000 x g for 20 seconds. Add 100 uL sterile, deionized water to achieve a final antibody concentration of 1 mg/ml. After reconstitution, the antibody will be in a 1X PBS solution. Aliquot and store at -80°C. Avoid freeze/thaw cycles.

12352203