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ProductsBack/Anti-Ubiquitin UBB Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded Human breast cancer, using the Antibody at 1:250 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast cancer, using the Antibody at 1:250 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast cancer, using the Antibody at 1:250 dilution.

Anti-Ubiquitin UBB Rabbit Monoclonal Antibody

M02848-3
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetUBB
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Ubiquitin UBB Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DIG-21
  • Gene ID7314
  • Target name
    UBB
  • Target description
    ubiquitin B
  • Target synonyms
    HEL-S-50, polyubiquitin-B, epididymis secretory protein Li 50, polyubiquitin B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP0CG47
  • Protein Name
    Polyubiquitin-B
  • Scientific Description
    Boster Bio Anti-Ubiquitin UBB Rabbit Monoclonal Antibody catalog # M02848-3. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Ma C, Wang X, He S, et al. Ubiquitinated AIF is a major mediator of hypoxia-induced mitochondrial dysfunction and pulmonary artery smooth muscle cell proliferation. Cell Biosci. 2022,12(1):9. doi: 10.1186/s13578-022-00744-3
    Read this paper
  • Wang DY, Mou YN, Du X, et al. Ubr1-mediated ubiquitylation orchestrates asexual development, polar growth, and virulence-related cellular events in Beauveria bassiana. Appl Microbiol Biotechnol. 2021,105(7):2747-2758. doi: 10.1007/s00253-021-11222-0
    Read this paper
  • Wang DY, Ren K, Tong SM, et al. Pleiotropic effects of Ubi4, a polyubiquitin precursor required for ubiquitin accumulation, conidiation and pathogenicity of a fungal insect pathogen. Environ Microbiol. 2020,22(7):2564-2580. doi: 10.1111/1462-2920.14940
    Read this paper

Datasheet

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MSDS

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Figure 1. Western blot analysis of Ubiquitin using anti-Ubiquitin antibody (PB9122). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ubiquitin antigen affinity purified polyclonal antibody (Catalog # PB9122) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ubiquitin at approximately 9KD. The expected band size for Ubiquitin is at 9KD.
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