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Anti-UBXN8 (N-term) Antibody

102-22428
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetUBXN8
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-UBXN8 (N-term) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB36379
  • Gene ID7993
  • Target name
    UBXN8
  • Target description
    UBX domain protein 8
  • Target synonyms
    D8S2298E, REP8, UBXD6, UBX domain-containing protein 8, Reproduction/chromosome 8, UBX domain-containing protein 6, rep-8 protein, reproduction 8 protein
  • Host
    Rabbit
  • Protein IDO00124
  • Protein Name
    UBX domain-containing protein 8
  • Scientific Description
    Rabbit Anti-UBXN8 (N-term) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UBXN8 using anti-UBXN8 antibody (A13427-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBXN8 antigen affinity purified polyclonal antibody (Catalog # A13427-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBXN8 at approximately 36 kDa. The expected band size for UBXN8 is at 31 kDa.
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