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Anti-UCHL3 Rabbit Monoclonal Antibody

ORB866709
Biorbyt
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetUCHL3
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-UCHL3 Rabbit Monoclonal Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    WB 1:500-1:2000IHC 1:50-1:200. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    26U65
  • Conjugate
    Unconjugated
  • Gene ID7347
  • Target name
    UCHL3
  • Target description
    ubiquitin C-terminal hydrolase L3
  • Target synonyms
    testicular tissue protein Li 221; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L3; ubiquitin thioesterase L3; ubiquitin thiolesterase; UCH-L3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15374
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase isozyme L3
  • Scientific Description
    Anti-UCHL3 Rabbit Monoclonal Antibody. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UCHL3 using anti-UCHL3 antibody (M05004-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UCHL3 antigen affinity purified monoclonal antibody (Catalog # M05004-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UCHL3 at approximately 26 kDa. The expected band size for UCHL3 is at 26 kDa.
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