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Anti-USP10 Antibody

144-07505
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetUSP10
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-USP10 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9100
  • Target name
    USP10
  • Target description
    ubiquitin specific peptidase 10
  • Target synonyms
    UBPO, ubiquitin carboxyl-terminal hydrolase 10, deubiquitinating enzyme 10, ubiquitin specific protease 10, ubiquitin thioesterase 10, ubiquitin thiolesterase 10, ubiquitin-specific-processing protease 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14694
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase 10
  • Scientific Description
    USP10 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, Rat liver tissue All lanes: USP10 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 88, 93 kDa Observed band size: 88 kDa
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Figure 1. Western blot analysis of USP10 using anti-USP10 antibody (A03786-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human SIHA whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP10 antigen affinity purified polyclonal antibody (Catalog # A03786-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP10 at approximately 110,130 kDa. The expected band size for USP10 is at 87 kDa.
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Immunohistochemical staining of human cerebral cortex, fallopian tube, prostate and stomach using Anti-USP10 antibody HPA006731 (A) shows similar protein distribution across tissues to independent antibody HPA006749 (B).
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