Figure 1. Western blot analysis of Uteroglobin using anti-Uteroglobin antibody (A02201-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysate, Lane 2: rat lung tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Uteroglobin antigen affinity purified polyclonal antibody (Catalog # A02201-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Uteroglobin at approximately 10KD. The expected band size for Uteroglobin is at 10KD.
Figure 1. Western blot analysis of Uteroglobin using anti-Uteroglobin antibody (A02201-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysate, Lane 2: rat lung tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Uteroglobin antigen affinity purified polyclonal antibody (Catalog # A02201-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Uteroglobin at approximately 10KD. The expected band size for Uteroglobin is at 10KD.
Anti-Uteroglobin/Scgb1a1 Antibody Picoband(r)
A02201-2
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityRat
TargetScgb1a1
Overview
- SupplierBoster Bio
- Product NameAnti-Uteroglobin/Scgb1a1 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID25575
- Target nameScgb1a1
- Target descriptionsecretoglobin family 1A member 1
- Target synonymsCC10; CCPBP; CCSP; clara cell phospholipid-binding protein; clara cells 10 kDa secretory protein; PCBB; PCB-binding protein; secretoglobin, family 1A, member 1 (uteroglobin); Ugb; uteroglobin; Uteroglobin (Clara cell secretory protein)
- HostRabbit
- IsotypeIgG
- Protein IDP17559
- Protein NameUteroglobin
- Scientific DescriptionBoster Bio Anti-Uteroglobin/Scgb1a1 Antibody Picoband® catalog # A02201-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityRat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203