Anti-VEGF [G6-31]

Absolute Antibody

Anti-VEGF [G6-31]

7

A VEGF phage ELISA was used to measure binding affinity of G6-31 to individual alanine-substituted VEGF mutants versus wild type VEGF. Crystals of G6-31 and the receptor binding fragment of human VEGF have been solved at 3.6 A (Fuh et al, 2005). Treatment of mice undergoing E2-induced PRLoma with G6-31 was used to block VEGF-A, causing reductions in the Ki-67-positive anterior cells, increases in TUNEL-positive anerior cells, and repair of the microvessel count by CD34-immunohistochemistry (Miyajima et al, 2010). Fluorescence quenching solution assay and SPR have been used to determine Kon and Koff at 25 degrees celsius (Lee et al, 2004).

ImmunoFluorescence, ELISA, Neutralisation/Blocking, Other Application

ELISA; crystallisation; block; SPR; IF

Research Use Only

Vascular endothelial growth factor A

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203