Figure 1. Western blot analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Marker Lane 2: mouse heart tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse kidney tissue lysates. Lane 6: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates. Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse NIH3T3 whole cell lysates. The membrane was incubated with rabbit anti-VEGF Receptor 2 antigen affinity purified polyclonal antibody (Catalog # A00901-3) at 1.0 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Figure 1. Western blot analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Marker Lane 2: mouse heart tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse kidney tissue lysates. Lane 6: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates. Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse NIH3T3 whole cell lysates. The membrane was incubated with rabbit anti-VEGF Receptor 2 antigen affinity purified polyclonal antibody (Catalog # A00901-3) at 1.0 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Anti-VEGF Receptor 2/Kdr Antibody Picoband(r)
A00901-3-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetKdr
Overview
- SupplierBoster Bio
- Product NameAnti-VEGF Receptor 2/Kdr Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID16542
- Target nameKdr
- Target descriptionkinase insert domain protein receptor
- Target synonyms6130401C07; fetal liver kinase 1; Flk; Flk-; Flk1; Flk-1; kinase NYK; Krd-1; Ly73; orv; protein-tyrosine kinase receptor flk-1; soluble vascular endothelial growth factor receptor 2; sVEGFR-2; vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor-3; VEGF; VEGF receptor-2; VEGFR; VEGFR2; VEGFR-2
- HostRabbit
- IsotypeIgG
- Protein IDP35918
- Protein NameVascular endothelial growth factor receptor 2
- Scientific DescriptionBoster Bio Anti-VEGF Receptor 2/Kdr Antibody Picoband® catalog # A00901-3. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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