Figure 1. Western blot analysis of VEGF/VEGF164 using anti-VEGF/VEGF164 antibody (A00045-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse HEPA1-6 whole cell lysates, Lane 3: rat C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF/VEGF164 antigen affinity purified polyclonal antibody (Catalog # A00045-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF/VEGF164 at approximately 27KD, 40KD. The expected band size for VEGF/VEGF164 is at 25KD.
Figure 1. Western blot analysis of VEGF/VEGF164 using anti-VEGF/VEGF164 antibody (A00045-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse HEPA1-6 whole cell lysates, Lane 3: rat C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF/VEGF164 antigen affinity purified polyclonal antibody (Catalog # A00045-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF/VEGF164 at approximately 27KD, 40KD. The expected band size for VEGF/VEGF164 is at 25KD.
Anti-VEGF/Vegfa/VEGF164 Antibody Picoband(r)
A00045-1
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse, Rat
TargetVegfa
Overview
- SupplierBoster Bio
- Product NameAnti-VEGF/Vegfa Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID22339
- Target nameVegfa
- Target descriptionvascular endothelial growth factor A
- Target synonymsV; vascular endothelial growth factor A; vascular permeability factor; Veg; Vegf; VEGF12; VEGF16; VEGF18; Vpf
- HostRabbit
- IsotypeIgG
- Protein IDQ00731
- Protein NameVascular endothelial growth factor A
- Scientific DescriptionBoster Bio Anti-VEGF/Vegfa/VEGF164 Antibody Picoband® catalog # A00045-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203