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Figure 1. Western blot analysis of VEGF/VEGF164 using anti-VEGF/VEGF164 antibody (A00045-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse HEPA1-6 whole cell lysates, Lane 3: rat C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF/VEGF164 antigen affinity purified polyclonal antibody (Catalog # A00045-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF/VEGF164 at approximately 27KD, 40KD. The expected band size for VEGF/VEGF164 is at 25KD.
Figure 1. Western blot analysis of VEGF/VEGF164 using anti-VEGF/VEGF164 antibody (A00045-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse HEPA1-6 whole cell lysates, Lane 3: rat C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF/VEGF164 antigen affinity purified polyclonal antibody (Catalog # A00045-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF/VEGF164 at approximately 27KD, 40KD. The expected band size for VEGF/VEGF164 is at 25KD.
Figure 1. Western blot analysis of VEGF/VEGF164 using anti-VEGF/VEGF164 antibody (A00045-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse HEPA1-6 whole cell lysates, Lane 3: rat C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF/VEGF164 antigen affinity purified polyclonal antibody (Catalog # A00045-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF/VEGF164 at approximately 27KD, 40KD. The expected band size for VEGF/VEGF164 is at 25KD.

Anti-VEGF/Vegfa Antibody

A00045-1
Boster Bio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse, Rat
TargetVegfa
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-VEGF/Vegfa Antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID22339
  • Target name
    Vegfa
  • Target description
    vascular endothelial growth factor A
  • Target synonyms
    L-VEGF, Vegf, Vpf, vascular endothelial growth factor A, long form, vascular permeability factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00731
  • Protein Name
    Vascular endothelial growth factor A, long form
  • Scientific Description
    Boster Bio Anti-VEGF/Vegfa/VEGF164 Antibody Picoband® catalog # A00045-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Zhu Y, Liu R, Zhao X, et al. VEGF overexpression in transplanted NSCs promote recovery of neurological function in rats with cerebral ischemia by modulating the Wnt signal transduction pathway. Neurosci Lett. 2024,824:137668. doi: 10.1016/j.neulet.2024.137668
    Read this paper
  • Zhu J, Li W, Jing J. Design, Synthesis and Anti-Melanoma Activity of Novel Annexin V Derivative with β(3)-Integrin Affinity. Int J Mol Sci. 2023,24(13). doi: 10.3390/ijms241311107
    Read this paper
  • Zhang L, Yin Y, Guo J, et al. Chronic intermittent hypobaric hypoxia ameliorates osteoporosis after spinal cord injury through balancing osteoblast and osteoclast activities in rats. Front Endocrinol (Lausanne). 2023,14:1035186. doi: 10.3389/fendo.2023.1035186
    Read this paper
  • Zhu J, Li X, Gao W, et al. Integrin Targeting Enhances the Antimelanoma Effect of Annexin V in Mice. Int J Mol Sci. 2023,24(4). doi: 10.3390/ijms24043859
    Read this paper
  • MasodKhooy MJ, Farasat M, Salehi Salmi M, et al. Combinatorial treatment with Silybum marianum essential oil enhances the therapeutic efficacy of a 5-fluorouracil base therapy for hepatocellular carcinoma. Phytother Res. 2023,37(5):1968-1985. doi: 10.1002/ptr.7716
    Read this paper
  • Hu H, Wang S, He Y, et al. The role of bone morphogenetic protein 4 in corneal injury repair. Exp Eye Res. 2021,212:108769. doi: 10.1016/j.exer.2021.108769
    Read this paper
  • Lin C, Zhang Y, Wang J, et al. The study of effect and mechanism of 630-nm laser on human lung adenocarcinoma cell xenograft model in nude mice mediated by hematoporphyrin derivatives. Lasers Med Sci. 2020,35(5):1085-1094. doi: 10.1007/s10103-019-02892-4
    Read this paper
  • Zhang Y, Liu Y, Zou J, et al. Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1α and autophagy in human osteosarcoma. Oncotarget. 2017,8(53):91134-91149. doi: 10.18632/oncotarget.19845
    Read this paper
  • Huang M, Li G, Pan T, et al. A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis. Oncotarget. 2016,7(36):57705-57713. doi: 10.18632/oncotarget.9531
    Read this paper
  • Sun L, Yuan Q, Xu T, et al. Pioglitazone, a Peroxisome Proliferator-Activated Receptor x03B3; Agonist, Ameliorates Chronic Kidney Disease by Enhancing Antioxidative Capacity and Attenuating Angiogenesis in the Kidney of a 5/6 Nephrectomized Rat Model. Cell Physiol Biochem. 2016,38(5):1831-40. doi: 10.1159/000443121
    Read this paper

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Figure 1. Western blot analysis of VEGF/VEGF164 using anti-VEGF/VEGF164 antibody (A00045-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse HEPA1-6 whole cell lysates, Lane 3: rat C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF/VEGF164 antigen affinity purified polyclonal antibody (Catalog # A00045-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF/VEGF164 at approximately 27KD, 40KD. The expected band size for VEGF/VEGF164 is at 25KD.
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