More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-VEGF/Vegfa Picoband(r) Antibody
Figure 1. Western blot analysis of Vegfa using anti-Vegfa antibody (A00045-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vegfa antigen affinity purified polyclonal antibody (Catalog # A00045-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vegfa at approximately 27KD. The expected band size for Vegfa is at 25KD.
Figure 1. Western blot analysis of Vegfa using anti-Vegfa antibody (A00045-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vegfa antigen affinity purified polyclonal antibody (Catalog # A00045-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vegfa at approximately 27KD. The expected band size for Vegfa is at 25KD.
Figure 1. Western blot analysis of Vegfa using anti-Vegfa antibody (A00045-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vegfa antigen affinity purified polyclonal antibody (Catalog # A00045-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vegfa at approximately 27KD. The expected band size for Vegfa is at 25KD.

Anti-VEGF/Vegfa Picoband(r) Antibody

A00045-2
Boster Bio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse, Rat
TargetVegfa
100 ug
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-VEGF/Vegfa Picoband(r) Antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID22339
  • Target name
    Vegfa
  • Target description
    vascular endothelial growth factor A
  • Target synonyms
    L-VEGF, Vegf, Vpf, vascular endothelial growth factor A, long form, vascular permeability factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00731
  • Protein Name
    Vascular endothelial growth factor A, long form
  • Scientific Description
    Boster Bio Anti-VEGF/Vegfa Picoband® Antibody catalog # A00045-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Li S, Zhu Z, Chen Z, et al. Network pharmacology-based investigation of the effects of Shenqi Fuzheng injection on glioma proliferation and migration via the SRC/PI3K/AKT signaling pathway. J Ethnopharmacol. 2024,328:118128. doi: 10.1016/j.jep.2024.118128
    Read this paper
  • Li Y, Fu J, Hou H, et al. Chondroitin sulfate-modified antiangiogenic peptide conjugate induces cell apoptosis via the mitochondria-mediated pathway to perform antitumor activity. Int J Biol Macromol. 2024,262(Pt 1):129671. doi: 10.1016/j.ijbiomac.2024.129671
    Read this paper
  • Zhu Y, Liu R, Zhao X, et al. VEGF overexpression in transplanted NSCs promote recovery of neurological function in rats with cerebral ischemia by modulating the Wnt signal transduction pathway. Neurosci Lett. 2024,824:137668. doi: 10.1016/j.neulet.2024.137668
    Read this paper
  • Li S, Liu W, Liu J, et al. Low-dose TNF-α promotes angiogenesis of oral squamous cell carcinoma cells via TNFR2/Akt/mTOR axis. Oral Dis. 2024,30(5):3004-3017. doi: 10.1111/odi.14802
    Read this paper
  • Zhu J, Li W, Jing J. Design, Synthesis and Anti-Melanoma Activity of Novel Annexin V Derivative with β(3)-Integrin Affinity. Int J Mol Sci. 2023,24(13). doi: 10.3390/ijms241311107
    Read this paper
  • Chang R, Su Y, Kong H, et al. Upregulation of SEMP1 Contributes to Improving the Biological Functions of Trophoblast via the PI3K/AKT Pathway in Preeclampsia. Mol Biotechnol. 2024,66(3):531-543. doi: 10.1007/s12033-023-00774-3
    Read this paper
  • Zhang L, Yin Y, Guo J, et al. Chronic intermittent hypobaric hypoxia ameliorates osteoporosis after spinal cord injury through balancing osteoblast and osteoclast activities in rats. Front Endocrinol (Lausanne). 2023,14:1035186. doi: 10.3389/fendo.2023.1035186
    Read this paper
  • Zhu J, Li X, Gao W, et al. Integrin Targeting Enhances the Antimelanoma Effect of Annexin V in Mice. Int J Mol Sci. 2023,24(4). doi: 10.3390/ijms24043859
    Read this paper
  • MasodKhooy MJ, Farasat M, Salehi Salmi M, et al. Combinatorial treatment with Silybum marianum essential oil enhances the therapeutic efficacy of a 5-fluorouracil base therapy for hepatocellular carcinoma. Phytother Res. 2023,37(5):1968-1985. doi: 10.1002/ptr.7716
    Read this paper
  • Ma X, Li Z, Li T, et al. Long non-coding RNA HOTAIR enhances angiogenesis by induction of VEGFA expression in glioma cells and transmission to endothelial cells via glioma cell derived-extracellular vesicles. Am J Transl Res. 2017,9(11):5012-5021.
    Read this paper

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Biomatik
Vascular Endothelial Growth Factor A (VEGFA) Polyclonal AntibodyCAU30963
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetVegfa
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-VEGF [G6-31]AB01022-10.0-BT
ApplicationsImmunoFluorescence, ELISA, Neutralisation/Blocking, Other Application
ReactivityMouse
TargetVegfa
  • SizePrice
Product group Antibodies
GeneTex
VEGF164 + VEGF120 antibody [4F22]GTX52390
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityMouse
TargetVegfa
  • SizePrice
Figure 1. Western blot analysis of VEGF/VEGF164 using anti-VEGF/VEGF164 antibody (A00045-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse HEPA1-6 whole cell lysates, Lane 3: rat C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF/VEGF164 antigen affinity purified polyclonal antibody (Catalog # A00045-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF/VEGF164 at approximately 27KD, 40KD. The expected band size for VEGF/VEGF164 is at 25KD.
Product group Antibodies
Boster Bio
Anti-VEGF/Vegfa/VEGF164 Antibody Picoband(r)A00045-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetVegfa
  • SizePrice