Anti-VISTA [13F3]

Absolute Antibody

Anti-VISTA [13F3]

7

This antibody is specific for murine VISTA.

The specificity of this antibody has been confirmed in both ELISA and flow cytometry (Wang et al, 2011). This antibody has been used in flow cytometric analysis to assess VISTA expression in murine lupus models (Ceeraz et al, 2017; Sergent et al, 2018) and mice infected with HSV-1 (Srivastava et al, 2018). This antibody has been shown to block VISTA, and enhances T cell proliferation in vitro (Wang et al, 2011). In mice models, this antibody exacerbates experimental autoimmune encephalomyelitis (Wang et al, 2011) and murine arthritis and lupus disease progression (Sergent et al, 2018). The use of this antibody to achieve a VISTA blockade enhances anti-tumour immune responses in mice models of both transplantable and inducible melanoma, suppressing tumour growth (LeMercier et al, 2014).

Flow Cytometry, ELISA, Neutralisation/Blocking

ELISA; FC; blocking

Research Use Only

Monoclonal

13F3

Vsir

V-set immunoregulatory receptor

4632428N05Rik; Die; Dies1; differentiation of ESCs 1; PD-1; PD-1H; platelet receptor Gi24; V; VISTA; V-set domain-containing immunoregulatory receptor; V-type immunoglobulin domain-containing suppressor of T-cell activation

Mouse

IgG2b

V-type immunoglobulin domain-containing suppressor of T-cell activation

This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

This antibody was raised by immunising Armenian hamsters with EL4 cells overexpressing VISTA-RFP, and VISTA-Ig fusion protein.

-20°C,2°C to 8°C

12352203