Anti-VSIG4 [Nb119]

Absolute Antibody

Anti-VSIG4 [Nb119]

7

Nb119 was shown to exhibit an affinity for murine VSIG4 of 0.9 nM by SPR (Zheng et al, 2014) or 3.5 nM by ITC (Wen et al, 2017). It was further shown by flow cytometry that Nb119 recognised mouse VSIG4 expressed on liver macrophages. Nb119 was crystallised in complex with both murine and human VSIG4 (Wen et al, 2017).

Flow Cytometry, Western Blot, Other Application

WB; FC; SPR; crystallisation

Research Use Only

Monoclonal

Nb119

VSIG4

V-set and immunoglobulin domain containing 4

complement receptor of the immunoglobulin superfamily; CRIg; Ig superfamily protein; protein Z39Ig; V-set and immunoglobulin domain-containing protein 4; Z39IG

Rabbit

IgG

V-set and immunoglobulin domain-containing protein 4

This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Human, mouse

The extracellular domain of recombinantly-produced mouse VSIG4 was used to immunise an alpaca (Vicugna pacos). Peripheral blood lymphocytes isolated from the immunised alpaca were used to generate a Nanobody phage library, clones from which were selected for binding to the same recombinant mouse VSIG4 extracellular domain.

-20°C,2°C to 8°C

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