Anti-VSV-G [M55]

Absolute Antibody

Anti-VSV-G [M55]

9

This antibody reacts with a defined subsite within the major antigenic site of VSV-G. The exact epitope is not defined.

This stable, recombinant single-chain Fv-Ckappa (scFv-Ckappa) antibody fragment was generated by protoplast fusion of J558L myeloma cells with Escherichia coli K803 transformed with an appropriate scFv-Ckappa expression construct. The aptly named M55 scFv-Ckappa expression construct - containing a CDR2 Ser55-to-Arg substitution (T-to-G transition at the third base), found in the VH chain of several hypermutated antibodies (VI22, VI43, and VI53) - was derived from the secondary antibody clone VI24. M55 binds a defined subsite within the major antigenic site of VSV-G. However, the exact binding site in the subunit is not defined. M55 binding to VSV-G was measured using solid-phase ELISA. The VH Ser55-to-Arg substitution in M55, which is not found in its parental clone VI24, increased monovalent binding by about 10-fold compared with M0 (the germline derived scFv-Ckappa). Furthermore, crosslinking with HRP labeled mouse anti-Ckappa antibodies improved M55 binding 10-fold (Kalinke et al., 2000; PMID: 10963674).

ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

M55

Rabbit

IgG

Glycoprotein G

Virus

-20°C,2°C to 8°C

12352203