Anti-VWF [NMC-4]

Absolute Antibody

Anti-VWF [NMC-4]

9

The binding characterization of this antibody was done using ELISA (Shima et al, 1985). This antibody was used in the initial characterization of the three distinct functional sites on the von Willebrand factor molecule (PMID: 2477370). This antibody can be used for the immunoprecipitation of the von Willebrand factor from a sample (PMID: 7679102). A study reported that this antibody was shown to inhibit its binding to GP Ib, completely abolished SIPA (sheer induced platelet aggregation) under high shear force, even in the presence of epinephrine. However, this antibody only partially inhibited SIPA under low shear force (PMID: 1360339). This antibody is also reported to completely inhibit vWF binding to platelet glycoprotein (GP) lb induced by either ristocetin or botrocetin at an IgG concentration of approximately 10 micrograms/mL, and also blocked binding of asialo-vWF to GP lb (PMID: 1984791).

ImmunoPrecipitation, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

NMC-4

VWF

von Willebrand factor

F8VWF, VWD, von Willebrand factor, Factor VIII related antigen, coagulation factor VIII VWF

Human

IgG1

von Willebrand factor

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

-20°C,2°C to 8°C

12352203