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Immunohistochemical staining of human hippocampus shows strong nuclear positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows strong nuclear positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows strong nuclear positivity in neuronal cells.

Anti-WAC Antibody

HPA036528
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetWAC
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WAC
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51322
  • Target name
    WAC
  • Target description
    WW domain containing adaptor with coiled-coil
  • Target synonyms
    BM-016; DESSH; PRO1741; WW domain-containing adapter protein with coiled-coil; Wwp4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BTA9
  • Protein Name
    WW domain-containing adapter protein with coiled-coil
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WAC using anti-WAC antibody (A04941-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human SK-OV-3 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse thymus tissue lysates, Lane 10: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WAC antigen affinity purified polyclonal antibody (Catalog # A04941-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WAC at approximately 85KD. The expected band size for WAC is at 85KD.
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