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Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Anti-WDR24 Antibody

HPA039506
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetWDR24
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WDR24 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84219
  • Target name
    WDR24
  • Target description
    WD repeat domain 24
  • Target synonyms
    C16orf21, JFP7, GATOR2 complex protein WDR24, GATOR complex protein WDR24, WD repeat-containing protein 24
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96S15
  • Protein Name
    GATOR2 complex protein WDR24
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WDR24 using anti-WDR24 antibody (A12216). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR24 antigen affinity purified polyclonal antibody (Catalog # A12216) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR24 at approximately 88 kDa. The expected band size for WDR24 is at 88 kDa.
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TargetWDR24
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Protein IDQ96S15
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IHC image of CSB-PA836297LA01HU diluted at 1:200 and staining in paraffin-embedded human ovarian tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
WDR24 AntibodyCSB-PA836297LA01HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetWDR24
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