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Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.

Anti-WDR46 Antibody

HPA043004
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetWDR46
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WDR46
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9277
  • Target name
    WDR46
  • Target description
    WD repeat domain 46
  • Target synonyms
    BING4; C6orf11; FP221; UTP7; WD repeat-containing protein 46; WD repeat-containing protein BING4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15213
  • Protein Name
    WD repeat-containing protein 46
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WDR46 using anti-WDR46 antibody (A12816-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR46 antigen affinity purified polyclonal antibody (Catalog # A12816-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR46 at approximately 70 kDa. The expected band size for WDR46 is at 68 kDa.
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