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ProductsBack/Anti-WDR5 [RAB-C223]

Anti-WDR5 [RAB-C223]

AB01707-1.1-BT
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ChIP Chromatin ImmunoPrecipitation, ELISA
Product group Antibodies
TargetWDR5
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-WDR5 [RAB-C223]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody has been used and recommended for the following methods: ELISA; IF; spiked IP; IP-MS; ChIP; FC.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ChIP Chromatin ImmunoPrecipitation, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-C223
  • Gene ID11091
  • Target name
    WDR5
  • Target description
    WD repeat domain 5
  • Target synonyms
    BIG3; BIG-3; BMP2-induced 3-kb gene protein; CFAP89; cilia and flagella associated protein 89; SWD3; SWD3, Set1c WD40 repeat protein, homolog; WD repeat-containing protein 5
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP61964
  • Protein Name
    WD repeat-containing protein 5
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WDR5 using anti-WDR5 antibody (A01910-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR5 antigen affinity purified polyclonal antibody (Catalog # A01910-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR5 at approximately 37 kDa. The expected band size for WDR5 is at 37 kDa.
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