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Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.

Anti-WDR7 Antibody

HPA051057
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetWDR7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WDR7 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23335
  • Target name
    WDR7
  • Target description
    WD repeat domain 7
  • Target synonyms
    TRAG, WD repeat-containing protein 7, TGF-beta resistance associated, TGF-beta resistance-associated protein TRAG, rabconnectin-3 beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4E6
  • Protein Name
    WD repeat-containing protein 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WDR7 using anti-WDR7 antibody (A12636-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR7 antigen affinity purified polyclonal antibody (Catalog # A12636-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR7 at approximately 160 kDa. The expected band size for WDR7 is at 164 kDa.
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