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Immunohistochemical staining of human colon shows moderate membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate membranous and cytoplasmic positivity in glandular cells.

Anti-WNT8B Antibody

HPA036570
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetWNT8B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WNT8B Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7479
  • Target name
    WNT8B
  • Target description
    Wnt family member 8B
  • Target synonyms
    protein Wnt-8b, epididymis secretory sperm binding protein, wingless-type MMTV integration site family, member 8B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ93098
  • Protein Name
    Protein Wnt-8b
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WNT8B using anti-WNT8B antibody (A07933-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT8B antigen affinity purified polyclonal antibody (Catalog # A07933-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT8B at approximately 40-50 kDa. The expected band size for WNT8B is at 39 kDa.
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