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Immunohistochemical staining of human colon shows moderate membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate membranous and cytoplasmic positivity in glandular cells.

Anti-WNT8B Antibody

HPA036570
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetWNT8B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-WNT8B
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7479
  • Target name
    WNT8B
  • Target description
    Wnt family member 8B
  • Target synonyms
    epididymis secretory sperm binding protein; protein Wnt-8b; wingless-type MMTV integration site family, member 8B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ93098
  • Protein Name
    Protein Wnt-8b
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WNT8B using anti-WNT8B antibody (A07933-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT8B antigen affinity purified polyclonal antibody (Catalog # A07933-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT8B at approximately 40-50 kDa. The expected band size for WNT8B is at 39 kDa.
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Anti-WNT8B Antibody Picoband(r)A07933-1-CARRIER-FREE
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The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA043432(WNT8B Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
Product group Antibodies
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WNT8B AntibodyCSB-PA043432
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TargetWNT8B
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Product group Antibodies
GeneTex
Wnt8b antibodyGTX128109
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TargetWNT8B
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Product group Antibodies
RayBiotech
Anti-WNT8B / Wnt 8b (C-term) Antibody119-12547
TargetWNT8B
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Atlas Antibodies
PrEST Antigen WNT8BAPREST80177
Protein IDQ93098
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Mouse ovary lysates probed with Wnt8b Polyclonal Antibody, Unconjugated (bs-6245R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
Bioss Antibodies
Wnt8b Polyclonal AntibodyBS-6245R
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TargetWNT8B
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WNT8B / Wnt 8b AntibodyLS-C682058
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TargetWNT8B
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WNT8B AntibodyPACO20920
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TargetWNT8B
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Anti-WNT8B AntibodyA46527
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