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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Anti-XAF1 Antibody

HPA057302
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetXAF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-XAF1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54739
  • Target name
    XAF1
  • Target description
    XIAP associated factor 1
  • Target synonyms
    BIRC4-binding protein; BIRC4BP; HSXIAPAF1; XIAPAF1; XIAP-associated factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6GPH4
  • Protein Name
    XIAP-associated factor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of XAF1 using anti-XAF1 antibody (A03432-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XAF1 antigen affinity purified polyclonal antibody (Catalog # A03432-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XAF1 at approximately 35 kDa. The expected band size for XAF1 is at 35 kDa.
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA764111LA01HU at dilution of 1:100
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