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Immunohistochemical staining of human testis shows distinct nuclear positivity in germ cells and Leydig cells.
Immunohistochemical staining of human testis shows distinct nuclear positivity in germ cells and Leydig cells.
Immunohistochemical staining of human testis shows distinct nuclear positivity in germ cells and Leydig cells.

Anti-XRCC5 Antibody

HPA025813
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetXRCC5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-XRCC5
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7520
  • Target name
    XRCC5
  • Target description
    X-ray repair cross complementing 5
  • Target synonyms
    KARP-1, KARP1, KU80, KUB2, Ku86, NFIV, X-ray repair cross-complementing protein 5, 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, Ku autoantigen, 80kDa, Ku86 autoantigen related protein 1, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), lupus Ku autoantigen protein p86, nuclear factor IV, thyroid-lupus autoantigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13010
  • Protein Name
    X-ray repair cross-complementing protein 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of KU80 using anti-KU80 antibody (PB9464). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KU80 antigen affinity purified polyclonal antibody (Catalog # PB9464) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKU80 at approximately 83 kDa. The expected band size for KU80 is at 83 kDa.
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