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Immunohistochemical staining of human urinary bladder shows weak cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows weak cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows weak cytoplasmic positivity in urothelial cells.

Anti-XRN1 Antibody

HPA035006
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetXRN1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-XRN1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54464
  • Target name
    XRN1
  • Target description
    5'-3' exoribonuclease 1
  • Target synonyms
    SEP1, 5'-3' exoribonuclease 1, strand-exchange protein 1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IZH2
  • Protein Name
    5'-3' exoribonuclease 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of XRN1 using anti-XRN1 antibody (A02855-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRN1 antigen affinity purified polyclonal antibody (Catalog # A02855-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRN1 at approximately 200 kDa. The expected band size for XRN1 is at 194 kDa.
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