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Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.

Anti-YAF2 Antibody

HPA026867
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetYAF2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-YAF2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10138
  • Target name
    YAF2
  • Target description
    YY1 associated factor 2
  • Target synonyms
    YY1-associated factor 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IY57
  • Protein Name
    YY1-associated factor 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of YAF2 using anti-YAF2 antibody (A08678-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YAF2 antigen affinity purified polyclonal antibody (Catalog # A08678-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YAF2 at approximately 25 kDa. The expected band size for YAF2 is at 20 kDa.
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