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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Anti-YWHAG Antibody

HPA026918
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetYWHAG
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-YWHAG
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7532
  • Target name
    YWHAG
  • Target description
    tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
  • Target synonyms
    14-3-3 protein gamma; 14-3-3GAMMA; DEE56; EIEE56; KCIP-1; PPP1R170; protein kinase C inhibitor protein 1; protein phosphatase 1, regulatory subunit 170; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61981
  • Protein Name
    14-3-3 protein gamma
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of 14-3-3 Gamma/YWHAG using anti-14-3-3 Gamma/YWHAG antibody (A04148-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 Gamma/YWHAG antigen affinity purified polyclonal antibody (Catalog # A04148-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 14-3-3 Gamma/YWHAG at approximately 28KD. The expected band size for 14-3-3 Gamma/YWHAG is at 28KD.
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