Anti-Z-DNA/Z-RNA [Z22]

Absolute Antibody

Anti-Z-DNA [Z22]

7

Z22 binding to Z-DNA was evaluated by a competitive solid-phase ELISA (Moeller et al., 1982; PMID: 7118931). The binding affinity of Z22 Fab version engineered from the original mouse IgG2b format to Z-DNA by SPR was measured to have an apparent KD of ~160 nM and could be competed out by soluble brominated d(G-C)15 but not unmodified d(G-C)15 (B-DNA). Z22 scFv binds to the target with a similar affinity to Z22 Fab. Competitive ELISA was used to determine binding specificity. Gel retardation assays were also used to show Z22 binding to DNA-8-MOP adducts. Biotinylated Z22 was used to determine the distribution of Z-DNA in permeabilized, microbead-encapsulated nuclei after adding radioactive streptavidin, and compared to encapsulated permeabilized nuclei stained with DAPI (Wittig et al., 1989; PMID: 2921282). Z22 was shown to cross-react with Z-RNA (Zarling et al., 1990; PMID: 2153833). Z-RNA binding using this product (Ab00783-3.0) was shown using IF (Zhang et al., 2020; PMID: 32200799), and it was also used in FC (Koehler et al., 2021; PMID: 34192517). Furthermore, this product (Ab00783, unspecified isotype) was also used in IHC and IB (Yau et al., 2021; PMID: 34728780), and Electrophoretic Mobility Shift Assay (EMSA; gel mobility assay) (Li et al., 2022; PMID: 35744832).

Gel Shift Assay, Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, Other Application

Research Use Only

-20°C,2°C to 8°C

12352203