Anti-Z-DNA/Z-RNA [Z22], Mouse IgG1, kappa

Absolute Antibody

Anti-Z-DNA/Z-RNA [Z22], Mouse IgG1, kappa

9

Z22 binding to Z-DNA was evaluated by a competitive solid-phase ELISA (Moeller et al., 1982; PMID: 7118931). The binding affinity of Z22 Fab version engineered from the original mouse IgG2b format to Z-DNA by SPR was measured to have an apparent KD of ~160 nM and could be competed out by soluble brominated d(G-C)15 but not unmodified d(G-C)15 (B-DNA). Z22 scFv binds to the target with a similar affinity to Z22 Fab. Competitive ELISA was used to determine binding specificity. Gel retardation assays were also used to show Z22 binding to DNA-8-MOP adducts. Biotinylated Z22 was used to determine the distribution of Z-DNA in permeabilized, microbead-encapsulated nuclei after adding radioactive streptavidin, and compared to encapsulated permeabilized nuclei stained with DAPI (Wittig et al., 1989; PMID: 2921282). Z22 was shown to cross-react with Z-RNA (Zarling et al., 1990; PMID: 2153833). Z-RNA binding using this product (Ab00783-3.0) was shown using IF (Zhang et al., 2020; PMID: 32200799), and it was also used in FC (Koehler et al., 2021; PMID: 34192517). Furthermore, this product (Ab00783, unspecified isotype) was also used in IHC and IB (Yau et al., 2021; PMID: 34728780), and Electrophoretic Mobility Shift Assay (EMSA; gel mobility assay) (Li et al., 2022; PMID: 35744832). The rabbit IgG version of this antibody (Ab00783-23.0) was successfully used in a dot blot assay at a concentration of 2 microg/ml to detect Z-RNA in total cellular RNA extracted with TRIzol (Bae et al., 2025; PMID: 40425585).

Gel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, Other Application

Research Use Only

Monoclonal

Z22

Mouse

IgG1

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Other Species

-20°C,2°C to 8°C

41116161