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Immunohistochemical staining of human nasopharynx shows strong cytoplasmic and nuclear positivity in respiratory epithelial cells.
Immunohistochemical staining of human nasopharynx shows strong cytoplasmic and nuclear positivity in respiratory epithelial cells.
Immunohistochemical staining of human nasopharynx shows strong cytoplasmic and nuclear positivity in respiratory epithelial cells.

Anti-ZCCHC5 Antibody

HPA051991
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRTL3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ZCCHC5 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID203430
  • Target name
    RTL3
  • Target description
    retrotransposon Gag like 3
  • Target synonyms
    Mar3, Mart3, SIRH9, ZCCHC5, ZHC5, retrotransposon Gag-like protein 3, Sushi-Ichi retrotransposon homolog 9, mammalian retrotransposon-derived 3, zinc finger CCHC domain-containing protein 5, zinc finger CCHC-type containing 5, zinc finger, CCHC domain containing 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N8U3
  • Protein Name
    Retrotransposon Gag-like protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RTL3 using anti-RTL3 antibody (A31819). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTL3 antigen affinity purified polyclonal antibody (Catalog # A31819) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTL3 at approximately 50 kDa. The expected band size for RTL3 is at 53 kDa.
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