Anti-Zearalenone [QY1.5]

Absolute Antibody

Anti-Zearalenone [QY1.5]

7

This antibody is specific for zearalenone and show some cross reactivity with hydroxylated zearalenone analogs. It is a mycotoxin produced mainly by fungi belonging to the genus Fusarium in foods and feeds. Possess estrogenic activity in pigs, cattle and

The binding specificity of this scFv antibody towards mycotoxin zearalenone was determined using ELISA. The scFv version of this antibody was also able to detect zearalenone in spiked corn extract in a competitive-indirect ELISA (PMID: 8979354).

ELISA

ELISA

Research Use Only

Monoclonal

QY1.5

Rabbit

IgG

This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format for improved compatibility with existing reagents assays and techniques.

Fungus

Fusarium species

The original antibody was generated using phage display technology by panning an scFv library against zearalenone-ovalbumin conjugate. The scFv library was created by linking the heavy and light chain genes of antizearalenone hybridoma cell line (2G3-6E3-2E2) using DNA linker encoding peptide (Gly4Ser)3 and cloning into a phagemid (pCANTAB5E) to be expressed as a fusion protein on the surfaces of recombinant phages. The original hybridoma (2G3-6E3-2E2) was generated using zearalenone-BSA conjugate as an immunogen.

-20°C,2°C to 8°C

12352203