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Immunohistochemical staining of human ovary shows strong membranous and cytoplasmic positivity in oocyte.
Immunohistochemical staining of human ovary shows strong membranous and cytoplasmic positivity in oocyte.
Immunohistochemical staining of human ovary shows strong membranous and cytoplasmic positivity in oocyte.

Anti-ZP3 Antibody

HPA054061
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetZP3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ZP3
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7784
  • Target name
    ZP3
  • Target description
    zona pellucida glycoprotein 3
  • Target synonyms
    OOMD3; sperm receptor; zona pellucida glycoprotein 3B; zona pellucida glycoprotein ZP3; zona pellucida protein C; zona pellucida sperm-binding protein 3; Zp-3; ZP3A; ZP3A/ZP3B; ZP3B; ZPC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21754
  • Protein Name
    Zona pellucida sperm-binding protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ZP3 using anti-ZP3 antibody (A03543-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZP3 antigen affinity purified polyclonal antibody (Catalog # A03543-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZP3 at approximately 47 kDa. The expected band size for ZP3 is at 47 kDa.
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