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Antibodies

We offer one of the most comprehensive portfolios of antibodies. This includes monoclonal and polyclonal primary, secondary, conjugated, phospho-specific, functional, (isotype) controls, tagged and antibody pairs. In addition, we offer custom antibody services from several manufacturers.

The antibodies are generated in various hosts and react to antigens of different species like human, mouse, rat, rabbit or zebrafish. The antibodies are validated for multiple applications, including immunohistochemistry, western blot, immunoprecipitation, ELISA and flow cytometry, to ensure reliable performance for your research needs.

If you need a specific antibody and can’t find it in our webshop, please contact our technical support.

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Figure 1. Western blot analysis of MAP4K5 using anti-MAP4K5 antibody (A10186-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K5 antigen affinity purified polyclonal antibody (Catalog # A10186-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K5 at approximately 95KD. The expected band size for MAP4K5 is at 95KD.
Product group Antibodies
Boster Bio
TargetMAP4K5
  • SizePrice
Figure 1. Western blot analysis of MAP4K5 using anti-MAP4K5 antibody (A10186-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K5 antigen affinity purified polyclonal antibody (Catalog # A10186-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K5 at approximately 95KD. The expected band size for MAP4K5 is at 95KD.
Product group Antibodies
Boster Bio
TargetMAP4K5
  • SizePrice
Figure 1. Western blot analysis of MAP4K5 using anti-MAP4K5 antibody (A10186-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K5 antigen affinity purified polyclonal antibody (Catalog # A10186-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K5 at approximately 95KD. The expected band size for MAP4K5 is at 95KD.
Product group Antibodies
Boster Bio
TargetMAP4K5
  • SizePrice
Figure 1. Western blot analysis of MAP4K5 using anti-MAP4K5 antibody (A10186-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K5 antigen affinity purified polyclonal antibody (Catalog # A10186-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K5 at approximately 95KD. The expected band size for MAP4K5 is at 95KD.
Product group Antibodies
Boster Bio
TargetMAP4K5
  • SizePrice
Figure 1. Western blot analysis of MAP4K5 using anti-MAP4K5 antibody (A10186-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K5 antigen affinity purified polyclonal antibody (Catalog # A10186-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP4K5 at approximately 95KD. The expected band size for MAP4K5 is at 95KD.
Product group Antibodies
Boster Bio
TargetMAP4K5
  • SizePrice
Product group Antibodies
Boster Bio
TargetMAP4K5
  • SizePrice
Western Blot (WB) analysis of specific cells using PHF3 Polyclonal antibody.
Product group Antibodies
Boster Bio
TargetPHF3
  • SizePrice
Product group Antibodies
Boster Bio
TargetPHF3
  • SizePrice
Western blot analysis of extracts from Hela cells, using PHF3 antiobdy A10188.
Product group Antibodies
Boster Bio
TargetPHF3
  • SizePrice
Product group Antibodies
Boster Bio
TargetRAB6B
  • SizePrice
Figure 1. Western blotting validation for Anti-ERGIC2 Antibody A10196 Western blot analysis of extracts of various cell lines
Product group Antibodies
Boster Bio
TargetERGIC2
  • SizePrice
Figure 1. Immunohistochemistry validation of ARHGAP22 using Anti-ARHGAP22 Antibody (A10197). Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C
Product group Antibodies
Boster Bio
TargetARHGAP22
  • SizePrice