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Antibodies

We offer one of the most comprehensive portfolios of antibodies. This includes monoclonal and polyclonal primary, secondary, conjugated, phospho-specific, functional, (isotype) controls, tagged and antibody pairs. In addition, we offer custom antibody services from several manufacturers.

The antibodies are generated in various hosts and react to antigens of different species like human, mouse, rat, rabbit or zebrafish. The antibodies are validated for multiple applications, including immunohistochemistry, western blot, immunoprecipitation, ELISA and flow cytometry, to ensure reliable performance for your research needs.

If you need help finding the right antibodies for your research, please use our antibody request form.

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Western blot analysis of Raf1 expression in K562 cell lysate (M00446-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAF1 monoclonal antibody (Catalog # M00446-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAF1
Product group Antibodies
References
Boster Bio
Anti-Raf1 Rabbit Monoclonal AntibodyM00446-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRAF1
Western blot analysis of Raf1 expression in HeLa cell lysate (M00446). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAF1 monoclonal antibody (Catalog # M00446) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAF1
Product group Antibodies
Boster Bio
Anti-Raf1 Rabbit Monoclonal AntibodyM00446
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetRAF1
Western blot analysis of Insulin Receptor expression in HeLa cell lysate (M00447). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INSR monoclonal antibody (Catalog # M00447) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INSR
Product group Antibodies
Boster Bio
Anti-Insulin Receptor INSR Rabbit Monoclonal AntibodyM00447
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Rat
TargetINSR
Western blot analysis of PPAR gamma expression in (1) HeLa cell lysate; (2) PC-3 cell lysate; (3) THP-1 cell lysate.
Product group Antibodies
Boster Bio
Anti-PPAR gamma Rabbit Monoclonal AntibodyM00449
ApplicationsWestern Blot
ReactivityHuman
TargetPPARG
Western blot analysis of Lipocalin-2 expression in SW480 cell lysate (M00452). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCN2 monoclonal antibody (Catalog # M00452) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LCN2
Product group Antibodies
Boster Bio
Anti-Lipocalin-2 LCN2 Rabbit Monoclonal AntibodyM00452
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetLCN2
Western blot analysis of PAK1 expression in A431 cell lysate using anti-PAK1 antibody (M00454). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK1 antigen affinity purified polyclonal antibody (Catalog # M00454) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for PAK1.
Product group Antibodies
Boster Bio
Anti-PAK1 Monoclonal AntibodyM00454
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPAK1
Western blot analysis of beta 2 Microglobulin expression in U937 cell lysate.
Product group Antibodies
Boster Bio
Anti-beta 2 Microglobulin B2M Rabbit Monoclonal AntibodyM00456-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetB2M
Western blot analysis of Beta 2 Microglobulin using anti-Beta 2 Microglobulin antibody (M00456-2).
Product group Antibodies
Boster Bio
Anti-Beta 2 Microglobulin B2M Antibody Picoband(r) (monoclonal, 2H10)M00456-2
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Monkey
TargetB2M
Western blot analysis of ABCG2 expression in 293T cell lysate.
Product group Antibodies
Boster Bio
Anti-ABCG2/Bcrp Rabbit Monoclonal AntibodyM00457
ApplicationsWestern Blot
ReactivityHuman
TargetABCG2
IHC analysis of CD18 using anti-CD18 antibody (M00458-1). CD18 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-CD18 Antibody (M00458-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-CD18 ITGB2 Antibody Picoband(r) (monoclonal, 1A3/2A10)M00458-1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetITGB2
Boster Kit Box
Product group Antibodies
Boster Bio
Anti-Human CD18 DyLight(r) 550 conjugated ITGB2 Antibody(monoclonal, 1A3/2A10)M00458-Dyl550
ApplicationsFlow Cytometry
ReactivityHuman
TargetITGB2
Western blot analysis of CD18 expression in K562 cell lysate (M00458). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGB2 monoclonal antibody (Catalog # M00458) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGB2
Product group Antibodies
Boster Bio
Anti-CD18 ITGB2 Rabbit Monoclonal AntibodyM00458
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetITGB2
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