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Antibodies

We offer one of the most comprehensive portfolios of antibodies. This includes monoclonal and polyclonal primary, secondary, conjugated, phospho-specific, functional, (isotype) controls, tagged and antibody pairs. In addition, we offer custom antibody services from several manufacturers.

The antibodies are generated in various hosts and react to antigens of different species like human, mouse, rat, rabbit or zebrafish. The antibodies are validated for multiple applications, including immunohistochemistry, western blot, immunoprecipitation, ELISA and flow cytometry, to ensure reliable performance for your research needs.

If you need help finding the right antibodies for your research, please use our antibody request form.

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Western blot analysis of Phospho-HSL (S853) expression in (1) Mouse muscle lysate; (2) Mouse muscle lysate treated with AP (P06762-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIPE monoclonal antibody (Catalog # P06762-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIPE
Product group Antibodies
Boster Bio
Anti-Phospho-HSL (S853) Rabbit Monoclonal AntibodyP06762-1
Western blot analysis of Phospho-Hsp27 (S78) expression in (1) A431 cell lysate; (2) A431 cell lysate treated with Anisomycin (P00676). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB1 monoclonal antibody (Catalog # P00676) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Phospho-Hsp27 (S78) Rabbit Monoclonal AntibodyP00676
Western blot analysis of Phospho-IRE1 (S724) expression in (1) K562 cell lysate treated with AP; (2) Untreated K562 cell lysate.
Product group Antibodies
Boster Bio
Anti-Phospho-IRE1 (S724) Rabbit Monoclonal AntibodyP00683
Western blot analysis of p95/NBS1 phosphorylation expression in Jurkat cell lysate treated with Etopside (P00732). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NBN monoclonal antibody (Catalog # P00732) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NBN
Product group Antibodies
Boster Bio
Anti-Phospho-p95/NBS1 (S343) Rabbit Monoclonal AntibodyP00732
Western blot analysis of Phospho-PKC alpha (T638) expression in (1) Jurkat cell lysate treated with TPA; (2) Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-Phospho-PKC alpha (T638) Rabbit Monoclonal AntibodyP00743
Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-GSK3 beta (Ser9) Antibody(P00791-1) GSK3B was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GSK3B Antibody (P00791-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal AntibodyP00791-1
Western blot analysis of Phospho-ATF2 (T71) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Anisomycin.
Product group Antibodies
Boster Bio
Anti-Phospho-ATF2 (T71) Rabbit Monoclonal AntibodyP00916-3
Western blot analysis of Phospho-AMPK alpha 1 (S496) expression in (1) 293T cell lysate treated with LP; (2) 293T cell lysate (P00994-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAA1 monoclonal antibody (Catalog # P00994-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKAA1
Product group Antibodies
Boster Bio
Anti-Phospho-AMPK alpha 1 (S496) Rabbit Monoclonal AntibodyP00994-1
Western blot analysis of Phospho-POLR2A (S2) expression in MCF7 cell lysate treated with Lambda Phosphatase (P01029-1) (1); MCF7 cell lysate (2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2A monoclonal antibody (Catalog # P01029-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR2A
Product group Antibodies
Boster Bio
Anti-Phospho-POLR2A (S2) Rabbit Monoclonal AntibodyP01029-1
Western blot analysis of Phospho-POLR2A (S5) expression in (1) HeLa cell lysate treated with Lambda Phosphatase (P01029-2); (2) HeLa cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2A monoclonal antibody (Catalog # P01029-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Phospho-POLR2A (S5) Rabbit Monoclonal AntibodyP01029-2
Western blot analysis of Phospho-Paxillin (Y118) expression in (1) HeLa cell lysate; (2) HeLa cell treated with pervanadate.
Product group Antibodies
Boster Bio
Anti-Phospho-Paxillin (Y118) Rabbit Monoclonal AntibodyP01033
Western blot analysis of Phospho-RSK1(S380) expression in A431 cell lysate treated with EGF (P01058-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KA1 monoclonal antibody (Catalog # P01058-1) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS6KA1
Product group Antibodies
Boster Bio
Anti-Phospho-RSK1 (S380) Rabbit Monoclonal AntibodyP01058-1
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